| Lane | Athlete | Yr | Team | Seed |
|---|---|---|---|---|
| 1 | Haerr, Grace | Black | 5:20.0h | |
| 2 | Placzek, Lena | Black | 5:10.0h | |
| 3 | Maser, Shannon | Black | 5:05.0h | |
| 4 | Newhouse, Carolyn | Gold | 5:10.0h | |
| 5 | Sindt, Jordyn | Black | 5:15.0h | |
| 6 | Saliba, Cindy | Gold | 5:10.0h | |
| 7 | Trost, Katharina | Gold | 5:00.0h | |
| 8 | Breen, Kelly | Black | 5:10.0h | |
| 9 | Kascel, Brooklyn | Gold | 5:20.0h |